Biochemistry I Laboratory

CHEM4401

Syllabus (Fall 2009)

Links

Example Lab Report .pdf

Labs and Lab supplements:

Lab 1 - Units, Concentrations, Solutions & Dilutions pdf

Lab 2 - Pipetting & Scales .pdf

Lab 3 - Buffers & pH (supplement - Practical Buffer Preparation) pdf Remember lab coats, goggles and Lab Safety paperwork

Lab 4 - Photometry (Supplement/Spectrophotometer instructions) Also bring practical buffer prep supplement pdf

Lab 5 - Amino Acid Chromatography (lab manual supplement) pdf

Lab 6 - Protein Structure Analysis Please bring your laptop to Lab pdf

Lab 7 - Enzyme activity: Polyphenoloxidase (lab manual supplement) pdf

Lab 8 - Enzyme Kinetics Please bring your laptop or a portable memory drive to Lab pdf

Lab 9 - Enzyme Databases pdf Laboratory mid-term this week. Please bring your laptop to Lab. Assignments completed outside of lab will not be accepted.

Lab 10 - Analysis of Reducing Sugars pdf

Lab 11 - Nucleic Acids pdf

Lab 12 - Chromatographic Methods of Lipid Separation pdf

Lab 13 - Laboratory Clean up, Check out, Evaluations and Final Exam

Links:

Dr. Feig's IU bioinformatics website

BLAST Course

BAST Tutorial

National Center For Biotechnology Information (NCBI)

NCBI BLAST search web page

Expert Protein Analysis System (ExPASy)

Kyoto Encyclopedia of Genes and Genomes (KEGG) database

Protein Explorer

BRENDA protein kinetics database

Protein Data bank

ClustalW Protein Sequence Alignment tool

Format Converter (conversion of sequences for ConSurf analysis)

The Sequence Manipulation Suite

Webcutter

MDL Chime Molecular Graphics Page (Chime download for Protein Explorer)

 

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